Tacrolimus (FK506)

Tacrolimus (Prograf, FK506) is a macrolide immunosuppressant (MW 804) isolated from Streptomyces tsukubaensis. Tacrolimus inhibits cell-mediated and humoral immune responses. Tacrolimus binds to a cytoplasmic protein FK506-binding protein 12 (FKBP12) to create a complex that inhibits phosphatase activity of calcineurin. Tacrolimus, like Cyclosporin A, inhibits signal transduction pathways linked to the T-cell receptor for antigen at the level of JNK and p38 kinase. Adverse events include nephrotoxicity, neurotoxicity, and diabetogenicity, which correlate with trough levels of tacrolimus. Hypertension, hyperkalemia, and thrombotic microangiopathy have also been reported.

Department: Mass Spectrometry Lab
Methodology: HPLC and tandem mass spectrometry (MS/MS)

Sample:
• Whole blood (EDTA) lavender top – 250ul
Handling:
Whole blood – freeze and store at -20ºC until shipped
Shipping:
• Whole blood - Ship sample overnight with 3-4 lbs of dry ice using a guaranteed overnight courier.

Special Notes: This research test must be coordinated through the Mass Spectrometry Laboratory Director. Most research tests are performed on a batch basis; the laboratory will not perform single sample testing. Contact the Mass Spectrometry Laboratory Director for more information.

Turnaround Time and Test Cost: Contact the Mass Spectrometry Laboratory Director.