Cyclosporin A

Cyclosporin A is a cyclic peptide (MW 1202) derived from the fungus Tolypocladium inflatum Gams and has been approved as a primary immunosuppressant for more than 2 decades. The complex of Cyclosporin A and cyclophilin is now known to inhibit the phosphatase activity of calcineurin. By preventing calcineurin-mediated dephosphorylation, Cyclosporin A inhibits translocation of the nuclear factor of activated T cells (NFAT) family of transcription factors from the cytoplasm to the nucleus of activated T cells. In addition, Cyclosporin A blocks the JNK and p38 signaling pathways that are triggered by antigen recognition in T cells. Adverse effects of Cyclosporin include nephrotoxicity, systemic hypertension, gingival hyperplasia, and neurotoxicity.

Department: Mass Spectrometry Lab
Methodology: HPLC and tandem mass spectrometry (MS/MS)

Sample:
• Whole blood (EDTA) lavender top – 250ul
Handling:
Whole blood – freeze and store at -20ºC until shipped
Shipping:
• Whole blood - Ship sample overnight with 3-4 lbs of dry ice using a guaranteed overnight courier.

Special Notes: This research test must be coordinated through the Mass Spectrometry Laboratory Director. Most research tests are performed on a batch basis; the laboratory will not perform single sample testing. Contact the Mass Spectrometry Laboratory Director for more information.

Turnaround Time and Test Cost: Contact the Mass Spectrometry Laboratory Director.